- Writer:
- Nesta Pain
- Producer:
- Rex Tucker
- Music specially composed by:
- John Hotchkis
- Film cameraman:
- Len Newson
- Editor:
- Ron de Mattos
- Designer:
- Susan Spence
- Louis Pasteur:
- Hugh Davis
- Marie Pasteur:
- Margaret Barton
- Professor Colin:
- Oliver Burt
- Colin's assistant:
- Barry MacGregor
- Roux:
- Alan Edwards
- Chamberland:
- Leslie Smith
- M. Rossignol:
- George A. Cooper
- M. Biot:
- Bruno Barnabe
- Agricultural Society Member:
- Richard Huggett
- Agricultural Society Member:
- Harold G. Robert
- Agricultural Society Member:
- John Cabot
- Other parts played by:
- Richard Statman
- Other parts played by:
- Malcolm Watson
- Other parts played by:
- Charles M. Julian
- Other parts played by:
- Paul Bogdan
- Other parts played by:
- Roger Williams
- Other parts played by:
- Donald McCollum
