- Unknown:
- Richard Church
- Broadcasting By:
- Mollie Greenhalgh
- Produced By:
- Wilfrid Grantham
- Played By:
- Wyndham Milligan
- Played By:
- John Kidd
- Played By:
- Geoffrey Bond
- Unknown:
- Frank Tickle
- Unknown:
- Peter Hoar
- Unknown:
- Christopher Marlowe
- Jarman Verson:
- Brian Hayes
- John Marlowe:
- Baliol Holloway
- Catherine Marlowe:
- Megan Latimer
- Laurence Applegate:
- Frank Atkinson
- Mistress Chapman:
- Vivienne Chatterton
- Mistress Hunt:
- Mary Williams
- John Moore:
- Rupert Davies
- Christopher Marlowe:
- Laurence Payne
- Joan Moore:
- Elizabeth London
- Helen Hunt:
- Patricia Field
- Queen Elizabeth:
- Janet Burnell
- Sir Francis Walsingham:
- Norman Claridge
- Lady Audrey Walsingham:
- Monica MacLeod
- Eleanor Bull:
- Nan Marriott-Watson
- Robert Poley:
- Geoffrey Wincott
- Ingram Friser:
- Preston Lockwood
- Nicholas Skeres:
- William Fox
- George Chapman:
- Paul Daneman
