- Unknown:
- Eugene Scribe
- Unknown:
- Radiotelevisione Italiana
- Chorus-Master:
- Roberto Benaglio
- Conducted By:
- Tullio Serafin
- Marguerite de Valois, Queen of Navarre:
- Antonietta Pastori
- Valentine, daughter of the Comte de Saint-Bris:
- Anna Di Cavalieri
- Urbait, the Queen's page:
- Jolanda Gardino
- A lady-in-waiting:
- Bianca Furlai
- Raoul de Nangis a Protestant noble man:
- Giacomo Lauri Volpi
- Marcel, a Huguenot soldier, servant to Raoul:
- Nicola Zaccaria
- Catholic Noblemen: Comte de Nevers:
- Giuseppe Taddei
- Catholic Noblemen: Comte de Saint-Bris:
- Giorgio Tozzi
- Catholic Noblemen: De Tavannes:
- Dino Formichini
- Catholic Noblemen: De Cossé:
- Tommaso Frascati
- Catholic Noblemen: De Thore:
- Giorgio Tadeo
- Catholic Noblemen: De Retz:
- Guido Mazzini
- Catholic Noblemen: De Mexu:
- Nestore Catalani
- Bois-Rose, a Huguenot soldier:
- Tommaso Frascati
- A servant:
- Renato Ercolani
- A constable:
- Guido Mazzini
- A friar:
- Renato Ercolani
- Girls::
- Bianca Furlai
- Girls::
- Editta Amedeo
