- Unknown:
- Robert Louis Stevenson
- Music By:
- John Hotchkis
- Production By:
- Raymond Raikes
- Played By:
- Cecile Chevreau
- Played By:
- Belle Chrystall
- Unknown:
- Geoffrey Hodson
- Unknown:
- Geoffrey Lumsden
- Unknown:
- Molly Rankin
- Richard Enfield:
- Gordon Davies
- Mrs Williams:
- Molly Rankin
- Bessie, her daughter:
- Cecile Chevreau
- Mr Edward Hyde:
- Cyril Shaps
- Mr Utterson:
- Richard Williams
- Dr Hastie Lanyon:
- Manning Wilson
- Poole, Dr Jekyll's butler:
- James Thomason
- Dr Henry Jekyll:
- Cyril Shaps
- Sister Bathurst:
- Belle Chrystall
- Police Inspector Newcomen of Scotland Yard:
- George Merritt
- A housemaid:
- Mairhi Russell
- Mr Guest:
- Simon Lack
- Keable, Dr Lanyon's butler:
- Geoffrey Lumsden
